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EMB agar, first described by Holt-Harris and Teague, contained lactose and sucrose as sources of carbohydrates. Levine modified the medium by adding peptone and phosphate, removing sucrose from the formula, and increasing the lactose content.

EMB agar; başta Escherichia coli, Enterobacter, Klebsiella, Salmonella, Shigella ve Pseudomonas türleri olmak üzere birçok Gram-negatif bakteriyi izole etmek için kullanılır.

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bu besiyerinin levine tarafından modifiye edilmiş formu olan levine emb agar yukarıdaki özelliklere ilaveten candida albicans 'ın hızlı tanımlanmasında kullanılır.

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EMB Agar (Eosin Methylene Blue Agar) is recommended for the isolation and differentiation of gram negative enteric bacteria from clinical and nonclinical specimens.