Prediluted controls and diluted patient sera are added to separate wells, allowing any H (+)/K (+) ATPase antibodies present to bind to the immobilized antigen. Unbound sample is washed away, and an enzyme-labeled antihuman IgG conjugate is added to each well.

Test Code PCAB Parietal Cell Antibodies, IgG, Serum Important Note Order LABRFPCABS in Epic Performing Laboratory Mayo Clinic Laboratories in Rochester

Test Code APAR Parietal Cell Antibodies, IgG, Serum Performing Laboratory Mayo Clinic Laboratories in Rochester

Use Detection of gastric parietal cell antibodies of the IgG class in human sera. Autoantibodies to gastric parietal cells are found in approximately 90% of patients with pernicious anemia and in about 30% of first degree relatives of patients with pernicious anemia.

Test Code PCAB Parietal Cell Antibodies, IgG, Serum Important Note Cerner Order Name: Parietal Cell Antibody IgG-Mayo

Storage location: Spin blood, freeze 0.5 mL (minimum 0.3 mL) serum in a plastic tube affixed with a large Cerner label in the CPA freezer, Send Outs rack. Off-site collection: Spin, aliquot, and freeze serum.

Reference Values Negative: ≤20.0 Units Equivocal: 20.1-24.9 Units Positive: ≥25.0 Units

Evaluating patients suspected of having pernicious anemia or autoimmune-mediated deficiency of vitamin B12 with or without megaloblastic anemia. Supplies: Sarstedt Aliquot Tube, 5 mL (T914) Collection Container/Tube: Preferred: Serum gel. Acceptable: Red top. Submission Container/Tube: Plastic vial. Specimen Volume: 0.5 mL Serum.

A positive result only indicates the presence of antibody to H(+)/K(+) ATPase and does not necessarily indicate the presence of autoimmune disease or other diseases.

Test ID PCAB Parietal Cell Antibodies, IgG, Serum Useful For Evaluating patients suspected of having pernicious anemia or autoimmune-mediated deficiency of vitamin B12 with or without megaloblastic anemia