Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 95190) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the medium.

Dispense 5 ml of medium into tubes containing increasing concentrations of cyanocobalamin (Cat. No. 43107) standard or the unknown. The total volume of 10 ml per tube is adjusted by adding distilled water.

The concentration of vitamin B 12 in the test sample is calculated based on the interpretation of the standard curve. Lactobacillus species grow poorly on non-selective culture media and require special nutrients for their growth.

B12 Assay MediumNutriSelect Plus - Find MSDS or SDS, a COA, data sheets and more information.

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B3801-100G — Vitamin B12 assay medium, 100g. Restored in factory conditions. Guarantee 1 year. from Millipore. B2B order at Samplify FZCO.

MilliporeSigma® (Sigma-Aldrich) SKU: B3801-100G $146.42 (You save) Net 30 Terms Available

SDS details and previous versions (where available) Vitamin B12 Assay Medium Manufacturer Millipore Sigma/ Sigma Aldrich USA/ Fluka / RdH Laborchemikalien / Sial /SAFC Product code 82897 Revision date 2025 March 10 Language English

The medium supports the growth of Lactobacillus delbrueckii subsp. lactis ATCCTM 7830 when prepared in single strength and supplemented with cyanocobalamin (vitamin B 12).

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